- Primate stomach: antibodies against parietal cells. With urea-pretreatment (top) and without urea-pretreatment (bottom).
- Primate stomach: antibodies against mitochondria. With urea-pretreatment (top) and without urea-pretreatment (bottom).
- Screening test for detection of antibodies against parietal cells.
- Indications: forms of chronic atrophic gastritis, pernicious anemia, funicular myelosis, various autoimmune endocrinopathies such as Basedow's and Addison's diseases.
- Initial dilution 1 : 10; polyvalent conjugate anti-human IgAGM, FITC-labelled.
- Primate stomach is the standard substrate for detection of parietal cell antibodies. For titration, stomach tissue from rat or mouse is sufficient.
- With positive results the parietal cells show a course granular to clumpy fluorescence, and the surrounding areas are usually dark.
- Parietal cell antibodies (PCA) are often mixed up with antibodies against mitochondria (AMA) in microscopic analysis. The latter give an even fine granular fluorescence of the parietal cell cytoplasm, with the surrounding region showing a (weaker) reaction.
- For reliable differentiation of both types of antibody, a 30-minute pretreatment of the tissue sections with urea-glycine buffer (order no. ZF 1140-0101) is recommended.
- The cytomplasmic fluorescence of parietal cells resulting from PCA occurs with the same intensity with or without urea pretreatment. The typical pattern of mitochondrial antibodies is almost completely supressed by urea pretreatment, greatly facilitating PCA diagnostics.
- In some AMA-positive samples it is possible to detect PCA that are obscured by the AMA pattern in conventional tissue sections.
- The urea-pretreated tissue shows a significantly darker background, enabling specific fluorescence to be more easily and reliable identified.
- This BIOCHIP can be supplemented with additional substrates, for example, thyroid (thyroid peroxidase, thyroglobulin), pancreas (pancreas islets), adrenal gland (adrenal cortex), ovary (ovary antigens), testis (Leydig cells), and intrinsic factor.
- Incubated ELISA Anti-Parietal Cells.
- Monospecific detection of antibodies against parietal cells (PCA).
- Indications: forms of chronic atrophic gastritis, pernicious anemia, funicular myelosis, various autoimmune endocrinopathies such as Basedow's and Addison's diseases.
- Serum dilution 1 : 200; conjugate class anti-human IgG, POD-labelled.
- 3-point calibration, quantitative.
- Native antigen: H+/K+-ATPase, purified by affinity chromatography.
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