Autoantibodies against Thyroid Gland Antigene

Indirect Immunofluorescence Test: EUROPLUS™ Thyroid Gland (unfixed) and Thyroglobulin

: Thyroid gland, unfixed and thyroglobulin BIOCHIP: antibodies against thyroid microsomes and thyroglobulin.

Detection of antibodies against thyroid gland antigens.
Indications: Basedow's disease, Hashimoto autoimmune thyroiditis.
Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
Using unfixed thyroid tissue, two important thyroid-specific antibodies can be found: Autoantibodies against thyroid microsomes (MAb) give a granular staining in the cytoplasm of the follicle epithelium (target antigen: thyroid peroxidase, TPO). Autoantibodies against thyroglobulin (TAb) react with the colloid of all follicles of the thyroid tissue and cause a reticular fluorescence pattern.
With the thyroglobulin-coated BIOCHIP, autoantibodies against thyroglobulin (TG) can be confirmed monospecifically in one and the same test procedure.
This BIOCHIP Mosaic™ can be supplemented as required with further substrates, e.g. rat kidney, to achieve a differentiation of antibodies against thyroid microsomes from mitochondrial antibodies (AMA). For a serological diagnosis of autoimmune polyendocrinopathies, BIOCHIPs with frozen sections of pancreas, adrenal gland, ovary, testis and stomach can be added.

Monospecific determination of antibodies against thyroglobulin (TG) and thyroid peroxidase (TPO).
Indications: Basedow's disease, Hashimoto autoimmune thyroiditis.
Serum dilution 1 : 100; conjugate class anti-human IgG, AP-labelled.
Autoantibodies against thyroglobulin and thyroid peroxidase can be detected simultaneously and monospecifically.

Monospecific determination of antibodies against thyroglobulin (TG), thyroid peroxidase (TPO), and thyrotropin receptor (TSH-R).
Indications: Grave's disease, Hashimoto autoimmune thyroiditis.
Serum dilution 1 : 200 (exception: anti-TSH-R undiluted); conjugate class anti-human IgG, POD-labelled (anti-TSH-R: avidin-labelled).
3-point calibration (exception: anti-TSH-R, 5-point calibration).
The quantification is carried out according to international reference preparations (anti-TG: NIBSC 65/93; anti-TPO: NIBSC 66/387; anti-TSH-R: NIBSC 90/672).
Thyroglobulin/TSH-R: native antigen; thyroid peroxidase: recombinant antigen.

Monospecific detection of autoantibodies against thyroglobulin (TG), thyroid peroxidase (TPO) and thyrotropin receptor (TSH-R).
Specific detection of the thyroid antigen thyrotropin and the hormones free triiodothyronine (FT3), free thyroxine (FT4), thyrotropin (TSH), calcitonin.
Indications: Graves' disease, Hashimoto's thyroiditis, medullary thyroid carcinoma, thyroidal C-cell hyperplasia, therapy controls in hyper- and hypothyrosis.
Serum dilutions: 1:50 for anti-TG and anti-TPO (magnetic), 1:20 for anti-TG and anti-TPO (precipitation), undiluted for all remaining test kits.
5-point to 8-point calibration (quantitative).

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